Mouse Heat Shock 70kDa Protein 5 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Heat Shock 70kDa Protein 5 in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . HSPA5 present in samples reacts with an anti-Mouse HSPA5 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-HSPA5 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active HSPA5 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse HSPA5. Detection Target: Mouse Heat Shock 70kDa Protein 5 Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.054ng/mL Storage Conditions: Store all components at 4┬░C This Mouse HSPA5 activity assay is for the quantitative determination of Heat Shock 70kDa Protein 5 in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.